Composite

Part:BBa_K611013:Design

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-18)


pLacI Characterization Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2186
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


Design Notes

There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.


Source

Built from existing parts in the registry though BioBrick assembly.

References